Assistant Specialist

Assistant Specialist

Job Description:

The Huebner lab investigates human development and disease using CRISPR tools, multicellular models, and functional genomic screens. The research projects utilize human iPSC-based models and functional genomics to uncover the molecular basis of developmental processes and the origins of cellular transformation. The Specialist will be part of a team that performs complex experiments including genome scale screens, genetic interaction mapping, and genome engineering. The Specialist will assist in the design, execution, and analysis of the following experiments in support of these research projects: (1) targeted and genome-wide pooled genetic screens in human cells, (2) production/testing of lentiviral sgRNA libraries for use in mammalian cells, (3) implementation of cell-based assays including flow cytometry, luciferase reporter detection, and microscopy-based approaches, (4) next-generation sequencing including single cell sequencing and (5) data analysis. The Specialist is expected to demonstrate the capacity to work in a semi-independent manner and as part of a team, as well as disseminate their findings in lab meetings and other public settings.

Required Qualifications:

• A master's degree in a biology-centered field or a baccalaureate degree with three or more years of direct cancer/stem cell research experience.
• 2+ years' experience deriving and maintaining mammalian cell lines including human iPSCs and 3D organoids
• Previous experience using FACS analyzers and sorters
• Prior experience performing immunofluorescence and confocal microscopy
• Experience in the execution of CRISPR growth/FACS screens, including guide library preparation and analysis
• Ability to generate lentivirus and transducing mammalian cells
• Proficient in molecular cloning techniques such as restriction digests, Gibson cloning, and plasmid design
• Proficient use of Python, R, Adobe Illustrator, and GraphPad

Preferred Qualifications:

• Familiarity with stem cell/developmental biology
• Experience generating genetically engineered cell lines
• Processing, QC, and alignment of NGS data (bulk RNAseq, scRNA-seq, ATACseq)

Please apply online at https://aprecruit.ucsf.edu/JPF05804, with a CV, cover letter, and three references.

See Table 24B for the salary range for this position. A reasonable estimate for this position is $63,500-$70,700.

• 3 required (contact information only)

As a University employee, you will be required to comply with all applicable University policies and/or collective bargaining agreements, as may be amended from time to time. Federal, state, or local government directives may impose additional requirements.

The University of California is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, protected veteran status, or other protected status under state or federal law.

As a condition of employment, the finalist will be required to disclose if they are subject to any final administrative or judicial decisions within the last seven years determining that they committed any misconduct.

* "Misconduct" means any violation of the policies or laws governing conduct at the applicant's previous place of employment, including, but not limited to, violations of policies or laws prohibiting sexual harassment, sexual assault, or other forms of harassment, or discrimination, as defined by the employer.

* UC Sexual Violence and Sexual Harassment Policy

* UC Anti-Discrimination Policy

* APM - 035: Affirmative Action and Nondiscrimination in Employment